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THE REPRODUCTION IN THE OPISTOBRANCHS (ALBORAN SEA)

The opisthobranchs are simultaneous hermaphrodites. The genital atrium is, in general, in the front right part, which is the reason why the individuals more or less face each other for copulation and show this side.

Two specimens of Trapania hispalensiscopulating on the sponge Sarcotragus spinosulus. In the one on the left, a parasitic copepod is seen by transparency and between the gills its setting. (Accelerated sequence x3)

Three Felimare picta setting up the copula.

Two Felimare picta copulating

Sea hares are one of the exceptions to this form of copulation, having a channel that leads the sperm from the dorsal part, where the genital atrium is, to a kind of penis located in the right frontal part. The fact that the penis and vagina are separated allows for intercourse to happen in chain form.

Spermatic channel

Chain of three Aplysia copulating

In some species, the penis may be armed with spines, like the one in the photograph belonging to a dorid-dove of the species Tyrannodoris europaea, thus permitting them to find a type of support in the vagina of the other specimen, to avoid turbulence caused by water movement during intercourse.

Penis of Tyrannodoris europaea

 

Spicules of the penis  Dendrodoris limbata

 

The sperm exchange takes place simultaneously and this is collected in the so-called seminal receptacle. In the following video of two Trapania hispalensis copulating, one can observe the displacement of the seminal packages that start from the specimen located in the lower part.

The genitalia is located next to the genital atrium and is formed by the structures that we see in the following image. On the left a drawing with the different structures separated and on the right as it is arranged on the animal.

Parts of the genitalia of Dendrodoris grandiflora: 1-Atrio genital, 2-Conducto deferente-, 3- Región prostática del conducto deferente, 4- Glándula femenina, 5- Bolsa copulatriz, 6- Receptáculo , 7- Ampolla, 8- Conducto vaginal, 9 y 10- Conducto uterino , 11- Conducto hermafrodita

Before oviposition the eggs are fertilized and covered by different protective layers, to defend them from possible predators.

Cross section of a laying F. villafranca  where, apart from the protective cover, two eggs are observed in the state of four cells.

 

Cut another section of the same put. In this case with four eggs, the one on the top right in the state of two cells and the rest of four cells.

 

Felimare villafranca doing the putting. The large size of the eggs and their small number can be observed, which indicates a type of larval development.

 

The eggs are deposited directly on the substrate, usually forming spirals and there is no parental care. The size of the eggs and the number of eggs per capsule vary from one species to another (see table). The color, size and shape of the eggs laid varies between species.

Egg laying of: A-Okenia mediterranea , B-Trapania hispalensis, C-T. hispalensis, D-Diaphorodoris luteocincta, E-D. papillata, F-Crimora papillata, G-Polycera quadrilineata, H-F. villafranca.

 

Egg laying of: A-Felimare tricolor, B-Felimare picta, C-Felimare bilineata, D-Felimida purpurea, E-Cadlina pellucida, F-Aldisa banyulensis, G- Discodoris rosi, H-Dendrodoris limbata.

 

The number of eggs per laying varies and has an influence on the type of larval development. In planctotrophic species (which feed on plankton), for example.  Trapania hispalensis, the number of eggs is very high, small and with a shorter time between oviposition and hatching. The veligerous larvae, in this type of development, are almost transparent because they have practically no vitello. Those that present a lecitotrophic type development (they eat yolk), like   Dendrodoris limbata, , have a smaller number of eggs and are larger. The larvae are opaque due to the yolk. Finally, in the species with direct development, such as Felimare villafranca, the number of eggs is very small and the velige stage takes place inside the egg.

Plantotrophic veliger of  T. hispalensis

Lecitotrophic veliger of Dendrodoris limbata

Juvenile newly hatched from Felimare villafranca
Dendrodoris limbata shell

From the egg, after a varied period between some species and others, and which depends on factors such as temperature (at a higher temperature, shorter time), a swimming larva called veligera, planctotrophic or lecitotrophic development, or a small juvenile, direct development ( See table). The lecithotrophic larvae present a smaller veil and lose the shell quickly.

Velíger of F. bilineata (planctotrohic) breaking the egg

Lecitotrophic veliger of Dendrodoris limbata.

Felimare villafranca (direct development), just before, during and after leaving the egg.

The type of development, logically, has a direct influence on the distribution of the species. Those of planktotrophic development can spend more time in the water column and, therefore, travel long distances pushed by the currents, before the larvae perform the metamorphosis. The lecitotrophic species depend on the yolk for its feeding, which is why this limits its permanency in the plankton, and therefore diminishes its dispersion. The lowest range is that of direct development, when a juvenile egg hatches directly.

Youths, just a few days old, of Felimare villafranca. One can appreciate the incipient blue coloration and the network of defensive spicules on the mantle.

MORE INFORMATION

TABLE with the characteristics of the laying of some doridáceos of our littoral.

Species

Number of eggs per capsule

Average egg size (µm)

Embryonic period in days

Type of development
Temperature
F. fountandraui 1 110 13 18º
D. limbata 1 222 - - 18º
D. areolata 1 80 12 Planctotrophic 18º
F. picta 1-2 130 + de 17 18º
F. villafranca 1 250 - Direct 18º
F. purpurea 1 93 13 Planctotrophic 18º
C. pellucida 1 63 8 Planctotrophic 20º
F. bilineata 1 65 11 Planctotrophic 20º
F. cantabrica 1 85 - Planctotrophic 22º
F. tricolor 1 85 11 Planctotrophic 18º
Tayuva lilacina 1 73 10 Planctotrophic 22º
D. rosi 1 80 11 Planctotrophic 22º
A. banyulensis 1 - - Direct -
J. onubensis 1-2 63 9 Planctotrophic 22º
O. mediterranea 1 77 - Planctotrophic 22º
T. maculata 1 100 11 - 22º
T. hispalenses 1 83 - Planctotrophic 22º
T. lineata 1 - - - 22º
C. papillata 1 72 - Planctotrophic 22º
Paradoris indecora 1 180 - - 22º

PHOTOGRAPHS

Capsule of  Armina tigrina with two eggs.

Egg in a state of four cells.

Egg of F. villafranca.

Egg of F. villafranca.

Veliger of  T. hispalensis.

Veliger of D. limbata

BIBLIOGRAPHY

Granada coastline

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General

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LUIS SÁNCHEZ TOCINO